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B16-antibody-conjugated-to-peptide-via-uv-cleavable-linker-tag-mass

B16-antibody-conjugated-to-peptide-via-uv-cleavable-linker-tag-mass

Rémi Lemaire, Isabelle Fournier, Michel Salzet, Michel Deschamps, Jean-Claude Tabet, Gottfried Proess, Ivo Rudloff, Marc Lemaitre

EP 2 325 631 A1 — family: WO 2007/000669 A2, US 8,221,972 B2
https://patents.google.com/patent/EP2325631A1/en

Abstract

A conjugate A–X–B in which A is a peptide tag of known molecular weight, X a linker cleaved during sample desorption/ionisation — in practice a UV-photocleavable linker — and B an antibody binding specifically to the target.

Imaging the released tag instead of the target itself turns MALDI imaging into a targeted and multiplexable readout. The patent also claims the method for mapping a target peptide, protein, antigen or hapten in a tissue section.

Patent family

Publication numberOfficeKindPublished
EP 2 325 631 A1European Patent Office — divisionalApplication2011-05-25
WO 2007/000669 A2WIPOApplication2007-01-04
US 8,221,972 B2United StatesGranted2012-07-17
  • Priority date — 7 June 2005
  • Filing date — 7 June 2006
  • Status — To be confirmed
  • Applicants — CNRS, Université des Sciences et Technologies de Lille, Eurogentec SA
  • Research axis — Targeted imaging — mass-tag probes

Context

The Tag-Mass concept, published as Lemaire et al., Tag-mass: specific molecular imaging of transcriptome and proteome by mass spectrometry based on photocleavable tag. Twenty years later the same idea is being re-filed with modern chemistry in WO 2025/242912 and WO 2025/242913. Co-filed with Eurogentec, which synthesised the conjugates.

Abstract as published

The invention concerns a conjugate of formula A-X-B, wherein A is a peptide tag molecule of known molecular weight, X is a linker that is cleaved during sample desorption/ionization, and B is an antibody that binds specifically to said target molecule. The invention also relates to a method for determining at least one target peptide, protein, antigen or hapten map in a tissue section, comprising the use of the conjugate.