Viral Vector
At VVEDIT, our Viral Vector division is dedicated to the development and optimization of "defective" viral vectors, which are engineered to ensure that they cannot replicate within the host cell or spread into the extracellular environment. Our platform offers expertise in four key types of viral vectors:
- Adenoviral Vectors: Known for their ability to deliver strong, transient expression of the genetic insert, these vectors are ideal for applications requiring high levels of gene expression over a short duration. Despite their potent delivery, adenoviral vectors can trigger a significant inflammatory response, making them suitable for specific in vivo applications where short-term expression is needed.
- Lentiviral Vectors: These vectors integrate into the host genome, allowing for the stable, long-term expression of the inserted gene across multiple cell generations. This feature makes lentiviral vectors particularly valuable in creating stable cell lines for in vitro studies, ensuring that the gene of interest is consistently expressed.
- Adeno-Associated Viral Vectors (AAVs): AAVs are the most commonly used viral vectors for in vivo applications, renowned for their ability to maintain long-term gene expression without integrating into the host genome. Additionally, AAVs do not provoke significant inflammatory responses, making them ideal for long-term therapeutic applications. Our platform offers a variety of natural and synthetic AAV serotypes, which can be tailored to target specific cell subtypes, enhancing the precision and effectiveness of gene delivery.
At VVEDIT, we provide comprehensive services in viral vector production, from vector design and construction to large-scale production and quality control, ensuring that our clients receive high-quality, customized viral vectors for their research and therapeutic needs.
Genome Editing and Transgenesis
The Genome Editing and Transgenesis division at VVEDIT specializes in the use of cutting-edge genetic tools to modify the genome with high precision. Our platform is equipped with state-of-the-art technology to facilitate a wide range of genome editing and transgenesis services, tailored to meet the specific needs of our clients:
- CRISPR-Cas9 Tools: We offer a variety of CRISPR-Cas9 systems, including Cas9 (D10A), Hypa-Cas9, and dCas9, each designed for different genome editing applications such as gene deletion, insertion, CRISPR screening, and CRISPR activation/inhibition. Our expertise in CRISPR technology ensures that the genome editing process is both efficient and precise, minimizing off-target effects.
- Fluorescent Transgenes: To assess off-target expression and optimize gene targeting, we utilize viral vectors encoding fluorescent markers like GFP and mCherry. These tools allow for real-time visualization and tracking of gene expression in target cells, enhancing the accuracy of our genome editing and transgenesis efforts.
- Custom Insert Design: Our platform provides customized design and construction of genetic inserts, including the transgene and the promoter that regulates its expression. This service ensures that the inserted gene functions optimally within the target cell, whether for research purposes or therapeutic applications.
At VVEDIT, we are committed to advancing the field of genome editing and transgenesis by providing our clients with the highest quality tools and services, enabling groundbreaking research and the development of innovative genetic therapies.
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